Journal: BMC Plant Biology

Article Title: Identification of microRNAs in developing wheat grain that are potentially involved in regulating grain characteristics and the response to nitrogen levels

doi: 10.1186/s12870-020-2296-7

Figure Lengend Snippet: Length distribution of miRNAs in developing wheat grain of plants grown under two nitrogen application levels and sampled at 7, 17, and 27 DAA. HN-7, HN-17 and HN-27 represent grain at 7, 17, and 27 DAA under high nitrogen application levels, respectively. LN-7, LN-17 and LN-27 represent grain at 7, 17, and 27 DAA under low nitrogen application levels, respectively

Article Snippet: A SYBR PrimeScript miRNA RT-PCR Kit was used to perform qRT-PCR in a fluorescence detection system (TianGen Biotech, Beijing, China) following the manufacturer’s instructions.

Techniques:

Journal: BMC Plant Biology

Article Title: Identification of microRNAs in developing wheat grain that are potentially involved in regulating grain characteristics and the response to nitrogen levels

doi: 10.1186/s12870-020-2296-7

Figure Lengend Snippet: Venn diagram of differentially expressed miRNAs in different libraries at three grain developmental stages under high (HN) and low (LN) nitrogen treatment. HN-7, HN-17 and HN-27 represent grain at 7, 17, and 27 days after anthesis under high nitrogen application levels, respectively. LN-7, LN-17 and LN-27 represent grain at 7, 17, and 27 days after anthesis under low nitrogen application levels, respectively. The number outside (inside) the brackets represents the number of total (novel) differentially expressed miRNAs

Article Snippet: A SYBR PrimeScript miRNA RT-PCR Kit was used to perform qRT-PCR in a fluorescence detection system (TianGen Biotech, Beijing, China) following the manufacturer’s instructions.

Techniques:

Journal: BMC Plant Biology

Article Title: Identification of microRNAs in developing wheat grain that are potentially involved in regulating grain characteristics and the response to nitrogen levels

doi: 10.1186/s12870-020-2296-7

Figure Lengend Snippet: The major GO categories of “cellular component”, “biological process”, and “molecular function” for the predicted genes of all the differentially expressed miRNAs during grain development

Article Snippet: A SYBR PrimeScript miRNA RT-PCR Kit was used to perform qRT-PCR in a fluorescence detection system (TianGen Biotech, Beijing, China) following the manufacturer’s instructions.

Techniques:

Journal: BMC Plant Biology

Article Title: Identification of microRNAs in developing wheat grain that are potentially involved in regulating grain characteristics and the response to nitrogen levels

doi: 10.1186/s12870-020-2296-7

Figure Lengend Snippet: Conserved miRNAs differentially expressed in developing wheat grain and their predicted target gene functions

Article Snippet: A SYBR PrimeScript miRNA RT-PCR Kit was used to perform qRT-PCR in a fluorescence detection system (TianGen Biotech, Beijing, China) following the manufacturer’s instructions.

Techniques: Ubiquitin Proteomics, Activity Assay, Protein Binding, Binding Assay, Alternative Splicing

Journal: BMC Plant Biology

Article Title: Identification of microRNAs in developing wheat grain that are potentially involved in regulating grain characteristics and the response to nitrogen levels

doi: 10.1186/s12870-020-2296-7

Figure Lengend Snippet: Differentially expressed novel miRNAs associated with grain development and their target gene functions

Article Snippet: A SYBR PrimeScript miRNA RT-PCR Kit was used to perform qRT-PCR in a fluorescence detection system (TianGen Biotech, Beijing, China) following the manufacturer’s instructions.

Techniques: Cell Differentiation, Ubiquitin Proteomics, Protein Binding

Journal: BMC Plant Biology

Article Title: Identification of microRNAs in developing wheat grain that are potentially involved in regulating grain characteristics and the response to nitrogen levels

doi: 10.1186/s12870-020-2296-7

Figure Lengend Snippet: Relationships within a miRNA-gene-GO network for cell differentiation, seed development and response to heat stress as determined by Cytoscape. The red, green, and yellow colors indicate miRNAs, target gene functions and GO terms, respectively

Article Snippet: A SYBR PrimeScript miRNA RT-PCR Kit was used to perform qRT-PCR in a fluorescence detection system (TianGen Biotech, Beijing, China) following the manufacturer’s instructions.

Techniques: Cell Differentiation

Journal: BMC Plant Biology

Article Title: Identification of microRNAs in developing wheat grain that are potentially involved in regulating grain characteristics and the response to nitrogen levels

doi: 10.1186/s12870-020-2296-7

Figure Lengend Snippet: Differentially expressed miRNAs between HN and LN treatments and their predicted target gene functions

Article Snippet: A SYBR PrimeScript miRNA RT-PCR Kit was used to perform qRT-PCR in a fluorescence detection system (TianGen Biotech, Beijing, China) following the manufacturer’s instructions.

Techniques: Ubiquitin Proteomics, Alternative Splicing, Activity Assay

Journal: BMC Plant Biology

Article Title: Identification of microRNAs in developing wheat grain that are potentially involved in regulating grain characteristics and the response to nitrogen levels

doi: 10.1186/s12870-020-2296-7

Figure Lengend Snippet: Model for miRNAs and their target genes associated with grain development and the response to N levels. The black letters in green circles indicate miRNAs, and the red letters in black boxes indicate the target gene function. The black arrows represent the direction of regulation. TF, transcription factor; CRF, cytokinin response factor; SCF, Skp-cullin-F-box; GLU, glucosyltransferase; BRI, brassinosteroid insensitive; ASR, alternative splicing regulator; LRR-RPK, leucine-rich-repeat receptor-like protein kinase; K1P-PSS1, kinesin-1-like protein PSS1; ARF, auxin response factor; HNT, high-affinity nitrate transport; Redox, reduction-oxidation; HSP, heat-shock protein; POD, peroxidase

Article Snippet: A SYBR PrimeScript miRNA RT-PCR Kit was used to perform qRT-PCR in a fluorescence detection system (TianGen Biotech, Beijing, China) following the manufacturer’s instructions.

Techniques: Alternative Splicing

Journal: BMC Plant Biology

Article Title: Identification of microRNAs in developing wheat grain that are potentially involved in regulating grain characteristics and the response to nitrogen levels

doi: 10.1186/s12870-020-2296-7

Figure Lengend Snippet: Verification of the expression patterns of nine miRNAs present in developing wheat grain. The different lowercase letters above the columns indicate significant differences ( P < 0.05)

Article Snippet: A SYBR PrimeScript miRNA RT-PCR Kit was used to perform qRT-PCR in a fluorescence detection system (TianGen Biotech, Beijing, China) following the manufacturer’s instructions.

Techniques: Expressing

Journal: BMC Plant Biology

Article Title: Identification of microRNAs in developing wheat grain that are potentially involved in regulating grain characteristics and the response to nitrogen levels

doi: 10.1186/s12870-020-2296-7

Figure Lengend Snippet: Verification of the expression patterns of miRNA target genes in developing wheat grain. The different lowercase letters above the columns indicate significant differences ( P < 0.05)

Article Snippet: A SYBR PrimeScript miRNA RT-PCR Kit was used to perform qRT-PCR in a fluorescence detection system (TianGen Biotech, Beijing, China) following the manufacturer’s instructions.

Techniques: Expressing